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mouse astrocyte medium  (ScienCell)

 
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    Structured Review

    ScienCell mouse astrocyte medium
    Mouse Astrocyte Medium, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+astrocyte+medium/astrocyte+conditioned+medium/pmc05404867-83-30-33
    Average 90 stars, based on 1 article reviews
    mouse astrocyte medium - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Suspension:

    Article Title: A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Article Snippet: .. 100μL of inactive mouse astrocyte suspension P2 (M1800, ScienCell) was applied to the PLL/LM-coated coverslips and incubated at 37 0 C for 2 hours to promote attachment before addition of mouse astrocyte medium (ScienCell). ..

    Incubation:

    Article Title: A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Article Snippet: .. 100μL of inactive mouse astrocyte suspension P2 (M1800, ScienCell) was applied to the PLL/LM-coated coverslips and incubated at 37 0 C for 2 hours to promote attachment before addition of mouse astrocyte medium (ScienCell). ..



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    Co-localization of <t>mouse</t> <t>apoE</t> and apoE4 in CAA or plaques within the same brain parenchyma in 5XFAD/apoE m/4 mice. 10-month-old 5XFAD/apoE m/4 mice were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the plaques and % area of plaque covered by different apoE. c - d Representative images of co-staining for mouse apoE and apoE4 in parenchymal CAA and % area of parenchymal CAA covered by different apoE. Values connected by lines were measured from the same animals ( n = 9/group; * p < 0.05, *** p < 0.001, paired t -test)
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    Image Search Results


    Co-localization of mouse apoE and apoE4 in CAA or plaques within the same brain parenchyma in 5XFAD/apoE m/4 mice. 10-month-old 5XFAD/apoE m/4 mice were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the plaques and % area of plaque covered by different apoE. c - d Representative images of co-staining for mouse apoE and apoE4 in parenchymal CAA and % area of parenchymal CAA covered by different apoE. Values connected by lines were measured from the same animals ( n = 9/group; * p < 0.05, *** p < 0.001, paired t -test)

    Journal: Acta Neuropathologica Communications

    Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

    doi: 10.1186/s40478-015-0250-y

    Figure Lengend Snippet: Co-localization of mouse apoE and apoE4 in CAA or plaques within the same brain parenchyma in 5XFAD/apoE m/4 mice. 10-month-old 5XFAD/apoE m/4 mice were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the plaques and % area of plaque covered by different apoE. c - d Representative images of co-staining for mouse apoE and apoE4 in parenchymal CAA and % area of parenchymal CAA covered by different apoE. Values connected by lines were measured from the same animals ( n = 9/group; * p < 0.05, *** p < 0.001, paired t -test)

    Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

    Techniques: Staining

    Comparison between apoE co-localization in parenchymal and leptomeningeal CAA in the same 5XFAD/apoE m/4 brains. Brain sections from 10 months old 5XFAD/apoE m/4 animals were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the leptomeningeal CAA and % area of leptomeningeal CAA covered by different apoE ( n = 9/group). Scale bars, 50 μm. c % area of plaque, parenchymal and leptomeningeal CAA covered by mouse apoE. d % area of plaque, parenchymal and leptomeningeal CAA covered by human apoE4. Values connected by lines were measured from the same animals ( n = 8/group; ** p < 0.01, *** p < 0.001, One-way ANOVA repeated measures)

    Journal: Acta Neuropathologica Communications

    Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

    doi: 10.1186/s40478-015-0250-y

    Figure Lengend Snippet: Comparison between apoE co-localization in parenchymal and leptomeningeal CAA in the same 5XFAD/apoE m/4 brains. Brain sections from 10 months old 5XFAD/apoE m/4 animals were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the leptomeningeal CAA and % area of leptomeningeal CAA covered by different apoE ( n = 9/group). Scale bars, 50 μm. c % area of plaque, parenchymal and leptomeningeal CAA covered by mouse apoE. d % area of plaque, parenchymal and leptomeningeal CAA covered by human apoE4. Values connected by lines were measured from the same animals ( n = 8/group; ** p < 0.01, *** p < 0.001, One-way ANOVA repeated measures)

    Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

    Techniques: Staining

    Co-localization of mouse apoE and apoE4 in plaques within the same brain parenchyma in APPPS1-21/apoE m/4 mice. Brain sections from 85-day-old APPPS1-21/apoE m/4 animals ( n = 7) were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a Representative images and the % of plaques containing both mouse apoE and apoE4 (scale bar, 20 μm). b Representative images and the % of plaques containing only mouse apoE (scale bar, 20 μm)

    Journal: Acta Neuropathologica Communications

    Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

    doi: 10.1186/s40478-015-0250-y

    Figure Lengend Snippet: Co-localization of mouse apoE and apoE4 in plaques within the same brain parenchyma in APPPS1-21/apoE m/4 mice. Brain sections from 85-day-old APPPS1-21/apoE m/4 animals ( n = 7) were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a Representative images and the % of plaques containing both mouse apoE and apoE4 (scale bar, 20 μm). b Representative images and the % of plaques containing only mouse apoE (scale bar, 20 μm)

    Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

    Techniques: Staining

    ApoE levels in 85-day-old apoE m/4 and APPPS1-21/apoE m/4 brains. The cortices were homogenized in PBS, followed by 1 % Triton X-100 and 5 M guanidine and apoE levels were measured by ELISA. a - b Absolute concentrations and fractional distribution of mouse apoE and apoE4 in apoE m/4 mice ( n = 10). c - d Absolute concentrations and fractional distribution of mouse apoE and apoE4 in APPPS1-21/apoE m/4 mice ( n = 10; * p < 0.05; ** p < 0.01; *** p < 0.001, Two-way ANOVA for repeated measures followed by Bonferroni post-test). e - g Correlations of Aβ 42 with mouse apoE, apoE4 and Aβ 40 in the insoluble fraction of APPPS1-21/apoE m/4 cortices ( n = 10)

    Journal: Acta Neuropathologica Communications

    Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

    doi: 10.1186/s40478-015-0250-y

    Figure Lengend Snippet: ApoE levels in 85-day-old apoE m/4 and APPPS1-21/apoE m/4 brains. The cortices were homogenized in PBS, followed by 1 % Triton X-100 and 5 M guanidine and apoE levels were measured by ELISA. a - b Absolute concentrations and fractional distribution of mouse apoE and apoE4 in apoE m/4 mice ( n = 10). c - d Absolute concentrations and fractional distribution of mouse apoE and apoE4 in APPPS1-21/apoE m/4 mice ( n = 10; * p < 0.05; ** p < 0.01; *** p < 0.001, Two-way ANOVA for repeated measures followed by Bonferroni post-test). e - g Correlations of Aβ 42 with mouse apoE, apoE4 and Aβ 40 in the insoluble fraction of APPPS1-21/apoE m/4 cortices ( n = 10)

    Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

    Techniques: Enzyme-linked Immunosorbent Assay